Similar and specific immunotropic effects of sulfate-chloride sodium-magnesium mineral waters "Myroslava" and "Khrystyna" of Truskavets' spa in healthy female rats

Badiuk Nataliya S., Popovych Dariya V., Hrytsak Myroslava V., Ruzhylo Sofiya V., Zakalyak Nataliya R., Kovalchuk Galyna Y., Mel’nyk Oksana I., Żukow Xawery. Similar and specific immunotropic effects of sulfate-chloride sodium-magnesium mineral waters "Myroslava" and "Khrystyna" of Truskavets’ spa in healthy female rats. Journal of Education, Health and Sport. 2021;11(11):314-333. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2021.11.11.031 https://apcz.umk.pl/JEHS/article/view/JEHS.2021.11.11.031 https://zenodo.org/record/6274256 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2021; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 10.10.2021. Revised: 22.10.2021. Accepted: 30.11.2021. SIMILAR AND SPECIFIC IMMUNOTROPIC EFFECTS OF SULFATE-CHLORIDE SODIUM-MAGNESIUM MINERAL WATERS "MYROSLAVA" AND "KHRYSTYNA" OF TRUSKAVETS’ SPA IN HEALTHY FEMALE RATS Nataliya S. Badiuk1, Dariya V. Popovych2, Myroslava V. Hrytsak1,3, Sofiya V. Ruzhylo4, Nataliya R. Zakalyak4, Galyna Y. Kovalchuk4, Oksana I. Mel’nyk5, Xawery Żukow6 1SE Ukrainian Research Institute of Medicine of Transport, Odesa, Ukraine badiuk_ns@ukr.net 2IY Horbachevs’kyi National Medical University, Ternopil’, Ukraine darakoz@yahoo.com 3Scientific group of Balneology of Hotel&Spa Complex "Karpaty", Truskavets’ hrytsak.myroslava@gmail.com ira_barschyk@ukr.net 4Ivan Franko Pedagogical University, Drohobych, Ukraine 5Danylo Halyts’kyǐ National Medical University, L’viv, Ukraine omelnyk7@gmail.com 6Medical University of Bialystok, Bialystok, Poland xaweryzukow@gmail.com Background. Earlier in an experiment on rats, we showed that newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa has a significant modulating effect on the parameters of metabolism and the autonomic nervous and endocrine systems. In this study, conducted in line with the concepts of neuroendocrine-immune complex and functional-metabolic continuum, data on the immunomodulatory effects of these waters on the same rats. Materials and Methods. Experiment was performed on 50 healthy female Wistar rats 230-290 g divided into 4 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Rats of the second (control) group for 6 days administered a single tap water through the tube at a dose of 1,5 mL/100 g of body mass. The rats of the main groups received the water "Myroslava" and "Khrystyna". The object of the study were the immune parameters of the thymus, spleen and blood. Results. The method of discriminant analysis revealed 12 parameters, according to which the animals loaded with mineral waters differed significantly from both control and intact animals. Classification accuracy is 86%. However, the difference between the immunotropic effects of mineral waters of different mineralization concerns only 9 parameters. Conclusion. The newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa have both similar and specific immunomodulating effects on healthy old female rats with weekly use. This provides a basis for preclinical studies. Keywords: sulfate-chloride sodium-magnesium mineral waters, immunity, female rats